Structure conservation in cytochromes P450
نویسندگان
چکیده
منابع مشابه
Functional evolution and structural conservation in chimeric cytochromes p450: calibrating a structure-guided approach.
Recombination generates chimeric proteins whose ability to fold depends on minimizing structural perturbations that result when portions of the sequence are inherited from different parents. These chimeric sequences can display functional properties characteristic of the parents or acquire entirely new functions. Seventeen chimeras were generated from two CYP102 members of the functionally dive...
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Creating artificial protein families affords new opportunities to explore the determinants of structure and biological function free from many of the constraints of natural selection. We have created an artificial family comprising 3,000 P450 heme proteins that correctly fold and incorporate a heme cofactor by recombining three cytochromes P450 at seven crossover locations chosen to minimize st...
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Cytochromes P450 (CYP) are monoxygenase enzymes involved in biotransformations of exogenous compounds in detoxification processes, but also in intoxication by generation of reactive species. CYP3A4, the most abundant isoform present in human liver, is responsible for the metabolisation of more than 60% of drugs used in therapy, leading to unwanted drug-drug interactions. The knowledge and under...
متن کاملHuman cytochromes P450 in health and disease.
There are 18 mammalian cytochrome P450 (CYP) families, which encode 57 genes in the human genome. CYP2, CYP3 and CYP4 families contain far more genes than the other 15 families; these three families are also the ones that are dramatically larger in rodent genomes. Most (if not all) genes in the CYP1, CYP2, CYP3 and CYP4 families encode enzymes involved in eicosanoid metabolism and are inducible...
متن کاملCytochromes P450 in the bioactivation of chemicals.
The initial view that the cytochrome P450 enzyme system functions simply in the deactivation of xenobiotics is anachronistic on the face of mounting evidence that this system can also transform many innocuous chemicals to toxic products. However, not all xenobiotic-metabolising cytochrome P450 subfamilies show the same propensity in the bioactivation of chemicals. For example, the CYP2C, 2B and...
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ژورنال
عنوان ژورنال: Proteins: Structure, Function, and Bioinformatics
سال: 2004
ISSN: 0887-3585
DOI: 10.1002/prot.20354